丝裂素原激活的蛋白激酶通路与异常瘢痕形成

【英文篇名】Activation of p44/42 mitogen-activated protein kinase pathway involves in formation of hypertrophic scar and keloid

【作者中文名】毕洪森; 陈东明; 李健宁;

【作者英文名】BI Hong -sen; CHEN Dong-ming; LI Jian-ning. Department of Plastic Surgery; Peking University Third Hospital; Beijing100083; China;

【作者单位】北京大学第三医院成形科; 北京大学第三医院成形科 100083北京; 100083北京;

【文献出处】中华医学美学美容杂志, Chinese Journal of Medical Aesthetics and Cosmetology, 编辑部邮箱 2004年 05期

期刊荣誉：

【关键词】瘢痕; 丝裂素原激活的蛋白激酶; 成纤维细胞;

【英文关键词】Cicatrix; Mitogen-activated protein kinases; Fibro blasts;

【摘要】目的研究丝裂素原激活的蛋白激酶 (p4 4 / 4 2MAPK)在异常瘢痕 (瘢痕疙瘩和增生性瘢痕 )中的表达和激活情况 ,探讨该信号通路在瘢痕形成中的作用。方法用免疫印迹法 (Westernblot ting)和免疫组织化学法 ,分别检测异常瘢痕和正常皮肤的组织和成纤维细胞中 p4 4 / 4 2MAPK和磷酸化p4 4 / 4 2MAPK ,定量分析两者在各组中表达的差异。 结果组织及其成纤维细胞中 p4 4 / 4 2MAPK含量在异常瘢痕与正常皮肤差异无显著性 ,而磷酸化 p4 4 / 4 2MAPK含量 ,以及磷酸化 p4 4 / 4 2MAPK与p4 4 /4 2MAPK之比 (ratio)明显高于正常皮肤。瘢痕疙瘩和增生性瘢痕相比 ,成纤维细胞中磷酸化 p4 4 /4 2MAPK含量在瘢痕疙瘩明显高于增生性瘢痕。结论p4 4 / 4 2MAPK信号通路的活化在异常瘢痕形成中起作用。

【英文摘要】Objective To examine the expression of p44/42 mitogen -activated protein kinase (p44/42MAPK) and Phospho-p44/42MAPK in cicatrix (hy pertropic scars and keloids) and to aim at exploring the role of activated p44/ 42 MAPK pathway in development of cicatrix. Methods In or der to analyse the differences, 10 samples of normal skin (NS), hypertropic scar s (HS) and keloids (K) were collected, and then the extracted cytoplasmic protei ns from each tissue were examined by Western blotting for p44/42 MAPK and Phosp…