巢蛋白和神经元素3在人胚胎胰腺中的表达和分布

【英文篇名】Expression of nestin and neurogenin 3 in the human fetal pancreas

【作者中文名】郑宗梅; 陈东明; 李凌松; 李健宁; 沈丽; 陆爱丽; 王淑玲; 鲍卫汉;

【作者英文名】ZHENG Zong-mei~*; CHEN Dong-ming; LI Ling-song; LI Jian-ning; SHEN Li; LU Ai-li; WANG Shu-ling; BAO Wei-han.~*Department of Plastic Surgery; Peking University Third Hospital; Beijing 100083; China;

【作者单位】北京大学第三医院成形科; 北京大学干细胞中心; 北京大学第三医院成形科 100083江苏省徐州市第一人民医院; 100083;

【文献出处】中华外科杂志, Chinese Journal of Surgery, 编辑部邮箱 2005年 23期

期刊荣誉：

【关键词】胰腺; 胰岛; 干细胞; 巢蛋白; 神经元素3;

【英文关键词】Panceras; Islets of Langerhans; Stem cells; Nestin; Neurogenin 3;

【摘要】目的探讨胰腺干细胞标志巢蛋白(NESTIN)和神经元素3(NGN3)在人胚胎胰腺组织中的表达及其与内分泌细胞、导管细胞和外分泌细胞标志的共表达情况。方法以因病理因素行药物引产的12周~14周前人胚胎胰腺14例为材料,应用免疫荧光染色和逆转录聚合酶链反应(RT-PCR)方法检测NESTIN 和NGN3的表达情况,免疫荧光染色检测胰腺内分泌标志胰岛素、胰高血糖素和外分泌标志CK-PAN和CK-19。结果NESTIN和NGN3在 12~14周人胚胎胰腺组织均广泛表达。NESTIN和NGN3阳性细胞中均无胰岛素及胰高血糖素表达;在胰岛中未见到NESTIN与NGN3共表达,但在导管中可见共表达。RT-PCR结果显示12~14周人胚胎胰腺中均有NESTIN和NGN3的表达。结论NGN3和NESTIN在人胚胎胰腺未分化细胞中表达,而具有分泌功能的内分泌细胞无表达。

【英文摘要】Objective To examine the expression of nestin and neurogenin 3(Ngn3),the markers of pancreatic stem cells,in the human fetal pancreas. Methods The human fetal pancreas tissue of 12 and 14 weeks were examined for the expression of nestin and Ngn3 using the techniques of immunofluorescence dye and RT-PCR.Results Both nestin and Ngn3 expressed widely in 12 and 14 weeks before in human fetal pancreatic tissue.In these positive cells there was no co-expressing insulin or glucagon.There were nestin and Ngn3 co-ex…