国产膨体聚四氟乙烯植入大鼠皮下后的相关组织学变化

发布时间：2010-04-16　　　浏览次数：　　　来源：北医三院成（整）形外科

导读：国产膨体聚四氟乙烯植入大鼠皮下后的相关组织学变化。…………

【英文篇名】The study of bladder epithelial cells culture in vitro

【作者英文名】ZHANG Jie; LI Dong; LI Jian – ning. （Department of Plastic Surgery; Peking University 3rd Hospital; Beijing 100083; China）;

【作者单位】北京大学第三医院成形外科; 北京大学第三医院成形外科北京;

【文献出处】中国实用美容整形外科杂志, Chinese Journal of Practical Aesthetic and Plastic Surgery, 编辑部邮箱 2005年 04期

期刊荣誉：

【关键词】膀胱; 上皮细胞; 细胞培养;

【英文关键词】Bladder; Epithelial cells; Cell culture;

【摘要】目的探索组织工程化尿道黏膜类似组织体外培养条件。方法取雄性大耳白兔幼兔的膀胱壁组织,分别用Ⅱ型胶原酶和DispaseⅡ处理后,胰蛋白酶消化获得单细胞悬液,接种后用含有或不含有表皮生长因子的培养液培养并传代,动态观察细胞形态变化及生长增殖的不同情况,并对细胞进行超微结构观察。结果用Ⅱ型胶原酶处理的标本在有表皮生长因子存在的情况下,细胞生长良好,混杂少量的成纤维细胞,在体外可传10代以上,成活30天以上。结论Ⅱ型胶原酶处理的幼兔膀胱黏膜上皮细胞纯度较高,可在体外培养一定时间并保持增殖能力,表皮生长因子是必要条件。

【英文摘要】Objective To study the bladder epithelial cell cultural condition in vitro for tissue engineering urethra. Methods A patch of bladder tissue taken from male young big – ear hare was treated by collagenase Ⅱ and Dispase Ⅱ respectively, and then the treated tissue was digested by trypsin into single cell suspension. The cultured cells (with or without EGF) were investigated morphologically and viably. Results The cells treated bycolagenase Ⅱ and cultured with EGF grew excellent, with few fibroblast contaminat…

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