体内外不同环境下成纤维细胞丝裂素原激活蛋白激酶的变化

【英文篇名】Activities of mitogen-activated protein kinase in fibroblasts in different internal and external environment

【作者中文名】毕洪森; 陈东明; 李健宁; 赵霞; 李生;

【作者英文名】Bi Hong-sen; Chen Dong-ming; Li Jian-ning; Zhao Xia; Li Sheng; Department of Plastic Surgery; Peking University Third Hospital; Beijing 100083; China;

【作者单位】北京大学第三医院成形科; 北京大学第三医院成形科 北京市;

【文献出处】中国临床康复, Chinese Journal of Clinical Rehabilitation, 编辑部邮箱 2005年 26期

期刊荣誉：

【关键词】瘢痕疙瘩; 蛋白激酶类; 成纤维细胞;

【摘要】目的:观察脱离体内环境因素时成纤维细胞中信号蛋白表达及细胞生物学特性的变化。方法:瘢痕疙瘩和正常皮肤(作为阴性对照)均来自北京大学第三医院成形科手术患者各6例。取小块瘢痕疙瘩和正常皮肤组织,用组织块接种法进行成纤维细胞原代培养后,进行细胞传代。试验所用为第5～8代细胞。①评价信号蛋白的含量,用体外培养的瘢痕疙瘩和正常皮肤成纤维细胞中p44/42丝裂原活化蛋白激酶和磷酸化的p44/42丝裂原活化蛋白激酶的积分吸光度值。②评价信号蛋白的活化强度,用体外培养的瘢痕疙瘩和正常皮肤成纤维细胞中p44/42丝裂原活化蛋白激酶和磷酸化的p44/42丝裂原活化蛋白激酶的平均积分吸光度值。③评价信号蛋白的活化程度用活化率值(磷酸化的p44/42丝裂原活化蛋白激酶积分吸光度值÷p44/42丝裂原活化蛋白激酶积分吸光度值)。结果:①p44/42丝裂原活化蛋白激酶和磷酸化的p44/42丝裂原活化蛋白激酶平均积分吸光度值比较:瘢痕疙瘩成纤维细胞均低于正常皮肤成纤维细胞 (0.023±0.005和0.025±0.005,0.030±0.000和0.042±0.004,P<0.05)。②p44/42丝裂原活化蛋白激酶和磷酸化的p44/42丝…

【英文摘要】AIM: To investigate the changes of cellular biological characteristics and expression of signal protein in fibroblasts out of the internal environment. METHODS: Keloids and normal skins(negative controls) were obtained from 6 patients and 6 normal people who operated in the Department of Plastic Surgery, Peking University Third Hospital. Small pieces of keloids and normal skins were taken so primarily culture fibroblasts by means of inoculating tissue sections so as to subculture the fibroblasts. The cells …